Mycoplasma is the general name of a class of microorganisms, more than one kind. Our mycoplasma removal reagent is applicable in a certain range. So, if the mycoplasma that the customer needs to remove is not within the range of this reagent, the effect will be unobvious.
If an improper removal reagent had been used for a long time before, it will lead to mycoplasma tolerance in the environment, which makes mycoplasma more difficult to be removed. In this case, it's suggested to extend the treatment for 1-2 weeks, and then observe the effect.
Improper storage may lead to the inactivation of the reagent. The reagent color is yellowish green, when long-termly exposed to light, the color will change and it will be invalid to use. Shading from light is necessary for storage.
When the environment is seriously contaminated by mycoplasma, the cells will be contaminated soon again even just after the removal. In this case, it is necessary to control the mycoplasma of the environment before the removal in cells.
The properties of the surface of the culture vessel are very important for cell culture. The glycoprotein (negative) on the cell surface is easy to adsorb on the hydrophilic surface. PLL has a hydrophilic surface. Polycations will interact with the anions on the cells to produce a strong adhesive force, which can prevent the cells from stacking and help the cells to adhere well.
Poly-L-lysine that remains sterile after recovery can be reused 2-3 times. But in order to ensure the sterile environment of cell culture and avoid harmful substances produced by bacteria, it is generally not recommended to reuse it.
