HEPES is all known as N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, is a commonly used biological buffer. pKa (25 °C) is 7.5 and an effective buffer range of pH is 6.8-8.2. The greatest advantage of HEPES is that to maintain the pH range of a solution for a longer period of time in an open environment. HEPES cannot pass through biofilms. It is a chemical and enzymatic stable substance with limited effect on biochemical reactions, and the absorption of visible and ultraviolet light is extremely low. Therefore, it is generally considered that HEPES is an excellent biological buffer, and there is no toxic effect to the cells. The working concentration is 10-50 mM and the final concentration for cell culture is 10 mM.
• 238.3g/L HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
|Animal Origin Ingredient
|1500 ~ 2020
|2℃-8℃, 24 months；-20℃, long term.
|2～8℃ or -5～-20℃, shading light