1M HEPES Solution
Cat.No.:PB180325
Item | Size | Price | Qty |
---|---|---|---|
100mL | Inquire |
Product Introduction
HEPES is all known as N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, is a commonly used biological buffer. pKa (25 °C) is 7.5 and an effective buffer range of pH is 6.8-8.2. The greatest advantage of HEPES is that to maintain the pH range of a solution for a longer period of time in an open environment. HEPES cannot pass through biofilms. It is a chemical and enzymatic stable substance with limited effect on biochemical reactions, and the absorption of visible and ultraviolet light is extremely low. Therefore, it is generally considered that HEPES is an excellent biological buffer, and there is no toxic effect to the cells. The working concentration is 10-50 mM and the final concentration for cell culture is 10 mM.
Ingredient Statement
With
• 238.3g/L HEPES
Without
• D-Glucose
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications | |
Concentration | 1M |
Volume | 100mL |
Form | Liquid |
Bacteria Detection | Negative |
Fungi Detection | Negative |
Mycoplasma Detection | Negative |
Ingredient Statement | 238.3g/L HEPES |
PH | 7.2-7.4 |
Animal Origin Ingredient | Without |
Osmotic Pressure | 1500 ~ 2020 |
Green Features | Sustainable packaging |
Dissolvent | Ultrapure water |
Valid Period | 2℃-8℃, 24 months;-20℃, long term. |
Storage Condition | 2~8℃ or -5~-20℃, shading light |
Shipping Condition | Ice bag |
Related Products
Documents
Citations
LncRNA SNHG6 promotes G1/S-phase transition in hepatocellular carcinoma by impairing miR-204-5p-mediated inhibition of E2F1
IF: 8.756Author:Kai Chen, Yifu Hou, Rui Liao
Journal:Oncogene
DOI:10.1038/s41388-021-01671-2
Cited products: PB180325
Oncogenic Role of Long Noncoding RNAMALAT1 in Thyroid Cancer Progression through Regulation of the miR-204/IGF2BP2/m6A-MYC Signaling
IF: 10.183Author:Mao Ye, Shu Dong, Haitao Hou
Journal:Molecular Therapy-Nucleic Acids
DOI:10.1016/j.omtn.2020.09.023
Cited products: PB180325
MCM3AP-AS1 KD Inhibits Proliferation, Invasion, and Migration of PCa Cells via DNMT1/DNMT3 (A/B) Methylation-Mediated Upregulation of NPY1R
IF: 10.183Author:Xin Li, Jiancheng Lv, Shuai Liu
Journal:Molecular Therapy-Nucleic Acids
DOI:10.1016/j.omtn.2020.01.016
Cited products: PB180325
microRNA-186 in extracellular vesicles from bone marrow mesenchymal stem cells alleviates idiopathic pulmonary fibrosis via interaction with SOX4 and DKK1
IF: 8.079Author:Jing Zhou, Yang Lin, Xiuhua Kang
Journal:Stem Cell Research & Therapy
DOI:10.1186/s13287-020-02083-x
Cited products: PB180325
Krüppel-Like Factor 6 Silencing Prevents Oxidative Stress and Neurological Dysfunction Following Intracerebral Hemorrhage via Sirtuin 5/Nrf2/HO-1 Axis
IF: 5.702DNMT3B decreases extracellular matrix degradation and alleviates intervertebral disc degeneration through TRPA1 methylation to inhibit the COX2/YAP axis
IF: 5.955NLRC4 gene silencing-dependent blockade of NOD-like receptor pathway inhibits inflammation, reduces proliferation and increases apoptosis of dendritic cells in mice with septic shock
IF: 5.955Dexmedetomidine Ameliorates Postoperative Cognitive Dysfunction via the MicroRNA-381-Mediated EGR1/p53 Axis
IF: 5.682Author:Yiliang Wang, Ying Zhang, Dasheng Cai
Journal:Molecular Neurobiology
DOI:10.1007/s12035-021-02417-7
Cited products: PB180325
MicroRNA‐377 exerts a potent suppressive role in osteosarcoma through the involvement of the histone acetyltransferase 1‐mediated Wnt axis
IF: 6.513Inhibitory effect of MSH6 gene silencing in combination with cisplatin on cell proliferation of human osteosarcoma cell line MG63
IF: 6.513Author:Hucheng Liu, Jin Zeng, Bin Zhang
Journal:Journal Of Cellular Physiology
Cited products: PB180325
Reduction of miR-744 delivered by NSCLC cell-derived extracellular vesicles upregulates SUV39H1 to promote NSCLC progression via activation of the Smad9/BMP9 axis
IF: 8.44Author:Liming Gao, Qi Tian,Tong Wu
Journal:Journal of Translational Medicine
DOI:10.1186/s12967-020-02654-9
Cited products: PB180325
Silenced lncRNA H19 and up-regulated microRNA-129 accelerates viability and restrains apoptosis of PC12 cells induced by Aβ25-35 in a cellular model of Alzheimer’s disease
IF: 5.173Author:Yanyun Zhang, Hailan Bao, Li xia Dong
Journal:Cell Cycle
DOI:10.1080/15384101.2020.1863681
Cited products: PB180325
microRNA-9-5p regulates the mitochondrial function of hepatocellular carcinoma cells through suppressing PDK4
IF: 5.854Author:Tao Si, Xuejian Ning, Hongwei Zhao
Journal:Cancer Gene Therapy
DOI:10.1038/s41417-020-00253-w
Cited products: PB180325
Inhibition of microRNA‐129‐5p expression ameliorates ultraviolet ray‐induced corneal epithelial cell injury via upregulation of EGFR
IF: 6.513Author:Ying Yang, Bo Gong, Zhengzheng Wu
Journal:Journal Of Cellular Physiology
Cited products: PB180325
Retracted: S100A9 gene silencing inhibits the release of pro‐inflammatory cytokines by blocking the IL‐17 signalling pathway in mice with acute pancreatitis
IF: 5.295Author:Dongmei Wu, Shan Wang, Min Shen
Journal:Journal Of Cellular And Molecular Medicine
Cited products: PB180325
Retracted: MicroRNA-381-induced down-regulation of CXCR4 promotes the proliferation of renal tubular epithelial cells in rat models of renal ischemia reperfusion injury
IF: 4.48Author:Guihong Zheng, Xin Wen, YongJian Wang
Journal:Journal Of Cellular Biochemistry
Cited products: PB180325
Inhibition of microRNA-300 inhibits cell adhesion, migration, and invasion of prostate cancer cells by promoting the expression of DAB1
IF: 5.173Author:Lin Li, Jing Hao, Chengquan Yan
Journal:Cell Cycle
DOI:10.1080/15384101.2020.1823730
Cited products: PB180325
Over-expression of JAZF1 promotes cardiac microvascular endothelial cell proliferation and angiogenesis via activation of the Akt signaling pathway in rats with myocardial ischemia-reperfusion
IF: 5.173Author:Jie Shang, ZhiYong Gao, LiYan Zhang
Journal:Cell Cycle
DOI:10.1080/15384101.2019.1629774
Cited products: PB180325
Up-regulation of THY1 attenuates interstitial pulmonary fibrosis and promotes lung fibroblast apoptosis during acute interstitial pneumonia by blockade of the WNT signaling pathway
IF: 5.173Author:Lin Chen, Rongzhen Tang, Jia Ruan
Journal:Cell Cycle
DOI:10.1080/15384101.2019.1578144
Cited products: PB180325
microRNA-363-3p reduces endothelial cell inflammatory responses in coronary heart disease via inactivation of the NOX4-dependent p38 MAPK axis
IF: 5.955MicroRNA-488 inhibits progression of colorectal cancer via inhibition of the mitogen-activated protein kinase pathway by targeting claudin-2
IF: 5.282Author:Yongbing Wang, Quan Shi, Gang Li
Journal:AM J PHYSIOL-CELL PH
DOI:10.1152/ajpcell.00047.2018
Cited products: PB180325
MicroRNA-23a inhibits melanoma cell proliferation, migration, and invasion in mice through a negative feedback regulation of sdcbp and the MAPK/ERK signaling pathway
IF: 3.885Specific knockdown of HOXB7 inhibits cutaneous squamous cell carcinoma cell migration and invasion while inducing apoptosis via the Wnt/β-catenin signaling pathway
IF: 4.249Author:Dong Gao, Hongquan Chen
Journal:American Journal of Physiology-Cell Physiology
DOI:10.1152/ajpcell.00291.2017
Cited products: PB180325
Histone Deacetylase Sirtuin 2 Enhances Viability of Trophoblasts Through p65-Mediated MicroRNA-146a/ACKR2 Axis
IF: 2.924Author:Yingchun Yu, Xiaoqin An, Dongmei Fan
Journal:Reproductive Sciences
DOI:10.1007/s43032-020-00398-x
Cited products: PB180325
HBXIP promotes gastric cancer via METTL3-mediated MYC mRNA m6A modification
IF: 5.955Overexpression of miR-150 Inhibits the NF-κB Signal Pathway in Intervertebral Disc Degeneration through Targeting P2X7
IF: 2.208Effects of microRNA-211 on proliferation and apoptosis of lens epithelial cells by targeting SIRT1 gene in diabetic cataract mice
IF: 3.976MicroRNA-182 downregulates Wnt/β-catenin signaling, inhibits proliferation, and promotes apoptosis in human osteosarcoma cells by targeting HOXA9
IF: 0Dexmedetomidine inhibits mitochondria damage and apoptosis of enteric glial cells in experimental intestinal ischemia/reperfusion injury via SIRT3-dependent PINK1/HDAC3/p53 pathway
IF: 8.44Author:Qin Zhang,Xiaoming Liu, Qian Hu
Journal:Journal of Translational Medicine
DOI:10.1186/s12967-021-03027-6
Cited products: PB180325
Silencing LINC00294 Restores Mitochondrial Function and Inhibits Apoptosis of Glioma Cells under Hypoxia via the miR-21-5p/CASKIN1/cAMP Axis
IF: 7.31Author:Xiaogang Dong, Qin Pi, Anwaier Yuemaierabola
Journal:Oxidative Medicine and Cellular Longevity
Cited products: PB180325
Silencing Long Non-coding RNA Kcnq1ot1 Limits Acute Kidney Injury by Promoting miR-204-5p and Blocking the Activation of NLRP3 Inflammasome
IF: 4.755Author:JunTao Wang, Peng Jiao, XiaoYing Wei
Journal:Frontiers in Physiology
Cited products: PB180325
Effects of total glucosides of paeony on acute renal injury following ischemia-reperfusion via the lncRNA HCG18/miR-16-5p/Bcl-2 axis
IF: 3.152Author:Wei Zhao, Yuan Zhang, Mingzhu Zhang
Journal:Immunobiology
DOI:10.1016/j.imbio.2022.152179
Cited products: PB180325
Inhibition of MicroRNA-302c on Stemness of Colon Cancer Stem Cells via the CARF/Wnt/β-Catenin Axis
IF: 3.487Author:Yun Zhang, Hua Meng, Kun Guo
Journal:Digestive Diseases and Sciences
DOI:10.1007/s10620-020-06435-8
Cited products: PB180325
The exploration of quantum dot-molecular beacon based MoS2 fluorescence probing for myeloma-related Mirnas detection
IF: 16.874Author:Jing Jing Wang, Ying Liu, Zhou Ding
Journal:Bioactive Materials
DOI:10.1016/j.bioactmat.2021.12.036
Cited products: PB180325