DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times. The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500mg/L (high Glucose type), which has been widely used in cell culture. DMEM (High glucose) was widely used in fast growth, low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cell culture and vaccine production. DMEM (High glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 4500mg/L D-Glucose
•
4mM L-Glutamine
•
15mg/L Phenol Red
•
1mM Sodium Pyruvate
Without
• HEPES
•
NaHCO3
Matters Need Attention
1. This product is for research use only.
2. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration
1×
Volume
5×1L,1×10L,1×50L
Form
Powder
pH
7.2-7.4
Animal Origin Ingredient
Without
Green_Features
Sustainable packaging
Primary Cells
Human Bronchial Fibroblast, Human Liver Parenchymal, Rat Cardiomyocyte, Mouse Pulmonary Artery Fibroblast