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Supplements

Supplements refer to a number of cell growth supplements that developed to make cells grow better in certain specific environments. For example, L-Alanyl-L-Glutamine is an advanced cell culture supplement that can directly replace L-Glutamine in cell culture media. L-glutamine (Glutamine) is an essential nutrient element in cell culture, but it is unstable in solution and will spontaneously degrade to generate ammonia and pyroglutamic acid, of which ammonia is harmful to cells, whereas L-alanine Acyl-L-glutamine is very stable in aqueous solution and will not spontaneously degrade. During cell culture, the cells will gradually release a peptidase into the culture medium to hydrolyze L-alanyl-L-glutamyl into L-alanine and L-glutamine, then the cells absorb and utilize the two hydrolysis products. The process of cells access to L-alanyl-L-glutamyl hydrolysis is similar to the flow-plus culture strategy, where low levels of L-glutamine are continuously added to the culture medium, thus improving the utilization of L-glutamine and not generating excess ammonia, which is more conducive to cell growth. Another example is HAT, which is commonly used for the screening of hybridoma cells and cell cultivation.

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Advantages of Pricella® Supplements

  • Express host and label clearly
  • Purity and Bio-activity clearly

    High Purity:>95% as determined by SDS-PAGE.

    The high activity was verified at the cellular level.

  • Stable product quality

    High batch-to-batch consistency.

  • Sterile,Endotoxin:Less than 1.0 EU per μg by the LAL method.
  • Good specificity

    More similar to the natural conformation and modification.

FAQs

β-Mercaptoethanol is also known as 2-mercaptoethanol, thioglycol, etc., commonly abbreviated as ME、2-Me or β-ME. β-Mercaptoethanol, which contains the functional groups of ethanediol (HOCH2CH2OH) and dithioglycol (HSCH2CH2SH), plays an important role in cell growth. On the one hand, it can reduce the sulfur compounds in the serum to glutathione, induce cell proliferation and delay cell senescence, while avoiding the damage of cells to peroxide. In addition, it promotes mitogen response and DNA synthesis, and increases the transformation effect of plant lectins (PHA) on lymphocytes. It has been widely used in hybridoma technology, and is also being used on cells that are difficult to culture, such as THP-1.
DPBS (with calcium, magnesium, without phenol red) (REF: PB180331) is used for β-Mercaptoethanol configuration. The concentration of the finished solution is 55mM, which can be used at a proper amount according to the concentration required for specific cell culture.
ITS supplements are used to reduce the amount of fetal bovine serum (FBS) needed for routine maintenance and low-density attachment of many cell types. Originally, they are used in combination with RPMI 1640 and MEM. Now, they are commonly used in combination with other basal media to support multiple cell types in the presence of 2 to 4% FBS. Earle’s Balanced Salt Solution (EBSS)can be used for ITS configuration.