Supplements

Supplements refer to a number of cell growth supplements that developed to make cells grow better in certain specific environments. For example, L-Alanyl-L-Glutamine is an advanced cell culture supplement that can directly replace L-Glutamine in cell culture media. L-glutamine (Glutamine) is an essential nutrient element in cell culture, but it is unstable in solution and will spontaneously degrade to generate ammonia and pyroglutamic acid, of which ammonia is harmful to cells, whereas L-alanine Acyl-L-glutamine is very stable in aqueous solution and will not spontaneously degrade. During cell culture, the cells will gradually release a peptidase into the culture medium to hydrolyze L-alanyl-L-glutamyl into L-alanine and L-glutamine, then the cells absorb and utilize the two hydrolysis products. The process of cells access to L-alanyl-L-glutamyl hydrolysis is similar to the flow-plus culture strategy, where low levels of L-glutamine are continuously added to the culture medium, thus improving the utilization of L-glutamine and not generating excess ammonia, which is more conducive to cell growth. Another example is HAT, which is commonly used for the screening of hybridoma cells and cell cultivation.

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