Can poly-L-lysine (PLL) be reused?
Poly-L-lysine that remains sterile after recovery can be reused 2-3 times. But in order to ensure the sterile environment of cell culture and avoid harmful substances produced by bacteria, it is generally not recommended to reuse it.
Why can most primary cells be cultured with poly-L-lysine (PLL)-coated vessels to promote cell attachment?
The properties of the surface of the culture vessel are very important for cell culture. The glycoprotein (negative) on the cell surface is easy to adsorb on the hydrophilic surface. PLL has a hydrophilic surface. Polycations will interact with the anions on the cells to produce a strong adhesive force, which can prevent the cells from stacking and help the cells to adhere well.
Can poly-L-lysine (PLL) be reused?
Poly-L-lysine that remains sterile after recovery can be reused 2-3 times. But in order to ensure the sterile environment of cell culture and avoid harmful substances produced by bacteria, it is generally not recommended to reuse it.
