Poly-L-Lysine (PLL) is a Poly-cation polymerized from lysine monomer.With a relative molecular weight of 70,000-300,000, PLL can be used as the growth matrix of cell culture, which can promote cell adhesion and adhesion to the wall. PLL is a commonly used cell adhesion agent in cell culture. Polyl-l-lysine (PLL) generally has molecular weight of 70,000-150,000, 150,000-300,000 and ＞300,000. The larger the molecular weight, the stronger the adhesion, but the more difficult it is to completely dissolve. Polyl-l-lysine with molecular weight of 150,000 - 300,000 is commonly used in cell culture.
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
4. Do not use the product if the solution is turbid.
Poly-L-lysine that remains sterile after recovery can be reused 2-3 times. But in order to ensure the sterile environment of cell culture and avoid harmful substances produced by bacteria, it is generally not recommended to reuse it.
Why can most primary cells be cultured with poly-L-lysine (PLL)-coated vessels to promote cell attachment?
The properties of the surface of the culture vessel are very important for cell culture. The glycoprotein (negative) on the cell surface is easy to adsorb on the hydrophilic surface. PLL has a hydrophilic surface. Polycations will interact with the anions on the cells to produce a strong adhesive force, which can prevent the cells from stacking and help the cells to adhere well.