MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells. It can also be used for other types of cell culture after the formula is modified. ATCC's modified MEM medium contains non-essential amino acids, sodium pyruvate, and reduces the amount of sodium bicarbonate.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000mg/L D-Glucose
•
2mM L-Glutamine
•
1500mg/L NaHCO3
•
1mM Sodium Pyruvate
•
1X NEAA
•
10mg/L Phenol Red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.