Ham's F-12 Nutrient Mixture was designed by Ham in 1969 based on Ham's F-10 nutrient mixture, which was originally used for serum-free culture of CHO cells. Ham's F-12 is often used as the basic culture medium for serum free culture. At low serum levels, it is especially suitable for single cell culture and clonal culture. After adding serum, it is also widely used in the culture of cancer cells and primary cells, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts, and chicken embryonic cells. In addition, the DMEM/F12 medium obtained by mixing Ham's F-12 in equal volume with DMEM is more nutritious and widely used.
Ingredient Statement
With
• 1802mg/L D-Glucose
•
1mM L-Glutamine
•
1176mg/L NaHCO3
•
1.2mg/L Phenol Red
Without
• HEPES
•
Sodium Pyruvate
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration
1 ×
Volume
500mL
Form
Liquid
Bacteria Detection
Negative
Fungi Detection
Negative
Mycoplasma Detection
Negative
Endotoxin Content
< 3
pH
7.2-7.4
Animal Origin Ingredient
Without
Green_Features
Sustainable packaging
Primary Cells
Human Epidermal Keratinocyte, Mouse Epidermal Keratinocyte, Rat Epidermal Keratinocyte
