This kit leverages the principle of size exclusion chromatography, facilitating the separation of exosomes based on the differential molecular sizes of their constituents. It provides several key advantages, including simplicity, exceptional purity, and high recovery rates, making it particularly ideal for exosome isolation from large-volume samples. The isolated exosomes are highly versatile, suitable for a wide range of applications, including Western blot (WB) analysis, nanoparticle tracking analysis (NTA), nanoparticle flow cytometry for particle size characterization, electron microscopy, omics research, and functional studies in both cellular and animal models.
Matters Need Attention
1. New or previously used purification columns may exhibit small gaps between the top frit and the white agarose beads due to gel settling during storage and handling. This does not affect column performance. Simply press the top frit down to eliminate any gaps before use.
2. The purification column is stored in 20% ethanol. Prepare 20% ethanol freshly before use, filter it through a 0.2 μm membrane, and degas it via ultrasonication or vacuum to prevent bubble formation, which can impair separation efficiency.
3. Prior to each use, wash the column with sterile PBS equilibrated to room temperature. Freshly prepare the PBS, filter it through a 0.2 μm membrane, and degas it to prevent bubble formation that could compromise performance.
4. Ensure no bubbles are present in the column before or during use. Inspect carefully to avoid any impact on experimental outcomes.
5. While the column can be reused, performance may decline with multiple uses. It is recommended to limit reuse to a maximum of 5 times
6. For exosome separation intended for NGS or other omics analyses, use a new purification column for each sample to avoid cross-contamination.
