This kit employs a centrifugal column-based method for exosome isolation. The column matrix efficiently adsorbs impurities while excluding exosomes, enabling ultra-fast isolation with high recovery rates. The isolated exosomes are suitable for downstream applications such as Western blot (WB) analysis, nanoparticle tracking analysis (NTA), nano-flow cytometry, electron microscopy, omics research, and functional studies in cellular and animal models. Additionally, this kit effectively removes free exosome-labeling dyes, ensuring enhanced purification quality.