The Rat Saphenous Vein Smooth Muscle Cells Isolation and Culture Kit is specifically developed for the extraction of primary rat saphenous vein smooth muscle cells. Verified through standardized procedures, each 1 Test of this kit enables the acquisition of one flask of cells (T-25 culture flask), with a cell count exceeding 1×106 cells. When subcultured at a 1:2 ratio, the cells can undergo 5 passages, with the best cell state within the first 3 passages. Through immunofluorescence analysis, the cell purity (α-SMA positive rate) has been confirmed to exceed 90%.
Specifications
Concentration
1. Prior to formal experiments, it is recommended to conduct anatomical simulation training using 1-2 normal rats to familiarize operators with procedural workflows and improve tissue dissociation efficiency.<br/>2. Reagent preparation or dispensing must strictly adhere to aseptic technique protocols. After dispensing, seal the containers immediately with a sealing film, use them promptly to avoid repeated freeze-thaw cycles or contamination.
