The Rat Pulmonary Aorta Smooth Muscle Cells Isolation and Culture Kit is specifically developed for the extraction of primary rat pulmonary aorta smooth muscle Cells. Verified through standardized procedures, each 1 Test of this kit enables the acquisition of one flask of cells (T-25 culture flask) per 1 Test, with a cell count exceeding 1×106 cells. When subcultured at a 1:2 ratio, the cells can undergo 5 passages,with the best cell state within the first 3 passages.Through immunofluorescence analysis, the cell purity (α-SMA-positive rate) has been confirmed to exceed 90%.
Specifications
Concentration
1. Prior to formal experiments, it is recommended to conduct anatomical simulation training using 1-2 normal rats to familiarize yourself with operational procedures and improve tissue isolation efficiency.It is difficult to obtain materials from the pulmonary artery, so it is necessary to practice to find the position of the pulmonary artery before starting the formal experiment.<br/>2. Reagent preparation or aliquoting must strictly adhere to aseptic techniques. After dispensing, seal the containers immediately with a sealing film, use them promptly to avoid repeated freeze-thaw cycles or contamination.
