The Rat Cortex Neuron Cells Isolation and Culture Kit is specifically developed to extract primary Rat Cortex Neuron Cells. As validated, standard operation using this kit enables the acquisition of one flask of cells (T-25 culture flask) per 1 Test, with a cell count exceeding 1×106 cells. Through immunofluorescence analysis, the cell purity (β-Tubulin Ⅲ-positive rate) has been confirmed to exceed 90%.
Matters Need Attention
1. Prior to formal experiments, it is recommended to conduct anatomical simulation training using 1-2 normal rats to familiarize operators with procedural workflows and improve tissue dissociation efficiency.
2. During the entire tissue dissociation process, place the small dish containing the tissue on an ice tray/ice box (2-8℃) to maintain hypothermic conditions. Critical precautions: Monitor temperature rigorously to prevent ice crystal formation in tissues/liquids.
3. Reagent preparation or dispensing must strictly adhere to aseptic technique protocols. After dispensing, seal the containers immediately with a sealing film, and use them promptly to avoid repeated freeze-thaw cycles or contamination.
4. Cortex neuron cells are terminally differentiated cells that lack proliferative capacity and exhibit high fragility. Improper enzymatic digestion can readily induce cell injury. During cell seeding, it is recommended to calculate the seeding density based on experimental requirements and inoculate into corresponding culture vessels. The recommended conversion ratios are as follows: one T25 flask ≈ one 6 cm dish ≈ two wells of a 6-well plate ≈ five to six wells of a 12-well plate.
