The Rat Brain Microvascular Endothelial Cells Isolation and Culture Kit is specifically developed for the extraction of primary rat brain microvascular endothelial cells. As validated, standard operation using this kit enables the acquisition of one flask of cells (T-25 culture flask) per 1 Test, with a cell count exceeding 1×106 cells. When passaged at a 1:2 ratio, the cells can undergo 1-2 passages. Through immunofluorescence analysis, the cell purity (CD31-positive rate) has been confirmed to exceed 90%.
Matters Need Attention
1. Prior to formal experiments, it is recommended to conduct anatomical simulation training using 1-2 normal rats to familiarize operators with procedural workflows and improve tissue dissociation efficiency.
2. During the entire tissue dissociation process, place the small dish containing the tissue on an ice tray/ice box (2-8℃) to maintain hypothermic conditions. Critical precautions: Monitor temperature rigorously to prevent ice crystal formation in tissues/liquids.
3. Reagent preparation or dispensing must strictly adhere to aseptic technique protocols. After dispensing, seal the containers immediately with a sealing film, use them promptly to avoid repeated freeze-thaw cycles or contamination.
