The Rat Astrocyte Cells Isolation and Culture Kit is specifically developed to extract primary Rat Astrocyte Cells. As validated, standard operation using this kit enables the acquisition of one flask of cells (T-25 culture flask) per 1 Test, with a cell count exceeding 1×106 cells. When passaged at a 1:2 ratio, the cells can undergo 3-5 passages. Through immunofluorescence analysis, the cell purity (GFAP-positive rate) has been confirmed to exceed 90%.
Matters Need Attention
1. Prior to formal experiments, it is recommended to conduct anatomical simulation training using 1-2 normal rats to familiarize operators with procedural workflows and improve tissue dissociation efficiency.
2. During the entire tissue dissociation process, place the small dish containing the tissue on an ice tray/ice box (2-8℃) to maintain hypothermic conditions. Critical precautions: Monitor temperature rigorously to prevent ice crystal formation in tissues/liquids.
3. Reagent preparation or dispensing must strictly adhere to aseptic technique protocols. After dispensing, seal the containers immediately with a sealing film, and use them promptly to avoid repeated freeze-thaw cycles or contamination.
4. It is recommended to culture cells using non-TC-treated T25 flasks or 6 cm dishes for the initial planting of cells after enzy matic digestion. If unavailable, TC-treated T25 flasks can be substituted. If cell adhesion occurs (a normal phenomenon), gently tap the flask to detach the adhered cells and proceed with trypsinization. The T25 flasks used for coating and the vessels used for subsequent subcultures are all TC-treated vessels.
