The Rat Aortic Smooth Muscle Cells Isolation and Culture Kit is specifically developed for the extraction of primary rat Aortic Smooth Muscle Cells. As validated, standard operation using this kit enables the acquisition of one flask of cells (T-25 culture flask) per 1 Test, with a cell count exceeding 1×106 cells. When passaged at a 1:2 ratio, the cells can undergo 5 passages,with the best cell state within the first 3 passages.Through immunofluorescence analysis, the cell purity (α-SMA-positive rate) has been confirmed to exceed 90%.
Specifications
Concentration
1. Prior to formal experiments, it is recommended to conduct anatomical simulation training using 1-2 normal rats to familiarize operators with procedural workflows and improve tissue dissociation efficiency.<br/>2. Reagent preparation or dispensing must strictly adhere to aseptic technique protocols. After dispensing, seal the containers immediately with a sealing film, use them promptly to avoid repeated freeze-thaw cycles or contamination.
