MEM α was developed on the basis of MEM medium. Compared with MEM medium, NEAA, sodium pyruvate, zinc sulfate, VB12, biotin and ascorbic acid were added. It is widely used in suspension and adherent cell culture of various mammals. Mem α medium without nucleoside and deoxynucleoside is often used as medium for DG44 and other DHFR- cells.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1000mg/L D-Glucose
•
2mM L-Alanyl-L-Glutamine
•
2200mg/L NaHCO3
•
1mM Sodium Pyruvate
•
1X Nucleoside
•
10mg/L Phenol Red
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination during the culture.
