Hybridoma serum-free medium is a serum-free product developed by Pricella's R&D team. It is suitable for the high-density growth and stable protein expression of hybridoma and myeloma cells.
Matters Need Attention
1. Depending on the specific conditions of the cells, choose whether to adapt the culture medium directly or indirectly.
2. To ensure stable cell growth, dilute passage is performed when the cell expansion ratio is ≥ 1:7, and centrifugal passage is performed when the cell expansion ratio is <
1:7.
3. Dilute passage: Calculate the required cell suspension during passage according to the cell density, remove excess cell suspension, and replenish with fresh culture medium to reach the desired culture volume.
4. Centrifugal passage: Calculate the cell suspension required for passage according to the cell density, centrifuge at 100 × g-300 × g for 5 minutes, remove the supernatant, and resuspend the cells in fresh culture medium.
