Ham's F-12 Nutrient Mixture was designed by Ham in 1969 based on Ham's F-10 Nutrient Mixture, which was originally used for serum-free culture of CHO cells. Ham's F-12 is often used as the basal culture medium for serum-free culture. It is especially suitable for single cell culture and cloning culture under low serum content. After adding serum, it is also widely used in the culture of cancer cells and primary cells, such as rat hepatocytes, rat prostate epithelial cells, rat, chondrocytes rat myoblasts, chicken embryo cells, etc. In addition, after mixing equal volumes of Ham's F-12 and DMEM, the DMEM/F12 medium obtained is more nutritious and more widely used.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for various types of cell culture, but does not contain proteins, lipids or any growth factors. So this product should be used with serum or serum-free additives.
Ingredient Statement
With
• 1802mg/L D-Glucose
•
1mM L-Glutamine
•
1.2mg/L Phenol Red
Without
• HEPES
•
NaHCO3
•
Sodium Pyruvate
Matters Need Attention
1. This product is for research use only.
2. It is necessary to pay attention to the aseptic operation and avoid the contamination.
Specifications
Concentration
1×
Volume
5×1L,1×10L,1×50L
Form
Powder
pH
7.2-7.4
Animal Origin Ingredient
Without
Green_Features
Sustainable packaging
Primary Cells
Human Epidermal Keratinocyte, Mouse Epidermal Keratinocyte, Rat Epidermal Keratinocyte
