Grace's supplement medium for insect cells was originally designed for cell growth of Antherea eucalypti, and is widely used for the cell growth of Spodoptera frugiperda, such as Sf9 and Sf21 cells. Sf9 and Sf21 cells are commonly used in baculovirus expression vector system ( BEVS ) to express recombinant proteins. This product contains hydrolyzed milk protein, yeast powder, L-glutamine and sodium bicarbonate. In addition, this product needs to be used with serum or serum-free additives.
Ingredient Statement
With
• 600mg/L L-Glutamine
•
700 mg/L D-Glucose
•
350 mg/L NaHCO3
•
3303 mg/L Hydrolyze milk protein
3330 mg/L Yeast
Without
• HEPES
•
Phenol Red
Sodium Pyruvate
•
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination during the culture.