Advanced MEM is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, serum supplementation can be reduced by 50–90% with no change in cellular prolife ratio or morphology. Many cell lines do not need to be domesticated to use this medium. Cells successfully cultured in Advanced MEM, with no adaptation, include Vero, HK-2, Hep G2, Caco-2, NCTC Clone 929, Hela, U-87 MG, HMC3, PK-15, etc.
Advanced MEM medium can achieve the serum-reduced effect due to the addition of the following ingredients: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, bovine serum albumin and various trace elements (sodium selenite, ammonium metavanadate, copper sulfate and manganese chloride).
Matters Need Attention
1. Not all cells are suitable for reduced serum culture. Be sure to test the effect in a small amount before replacing it. If necessary, the FBS concentration should be optimized for each cell line to obtain maximum serum reduction.
2. This product has been filtered and sterilized. Pay attention to aseptic operation to avoid contamination.
3. To maintain the best use of this product, do not perform freeze-thaw treatment.
4. For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.
